Workshop: An Army Marches on Its Metabolism In Vitro
Monday, January 29, 12:45-13:45
Sponsored and delivered by
Alicia D. Henn, PhD MBA
The rapidly developing fields of immunometabolism and stem cell research are fundamentally changing the paradigm of good cell culture. In vitro studies often overlook the fact that cells are normally grown in the presence of 16-21% oxygen concentration (room air condition) instead of 1-11%, the concentrations experienced by human tissues. Such an excess oxygen induces acute stress responses in cells, modifying cell metabolism and function through critical cell signaling pathways. Traditional room air cell culture techniques mistakenly modify immune and stem cell functions in vitro, and engraftment in vivo. In our own studies, we have found that human bone marrow mesenchymal stromal cells (MSC) are more likely to senesce when exposed to room air conditions that are used in routine cell culture. We will also share preliminary data on human B cells and cord blood hematopoietic stem cell (CB-HSC) indicating that in vitro oxygen levels affect cell migration through the HIF-1a/CXCR4 pathway. In conclusion, cells need more than just pre-warming their growth medium in a water bath; they also need to be protected from supraphysiologic room air oxygen concentrations.